Pigment concentration

Cells for pigment analysis were collected by filtration until clogging onto 0.7-µm GF/F filters. The pigments were extracted from the filters with 95% MeOH and measured by HPLC as described in Fournier et al. (2021), and were attributed to specific taxonomic groups according to Roy et al. (2011). Fournier, I. B., Lovejoy, C., and Vincent, W. F. (2021). Changes in the Community Structure of Under-Ice and Open-Water Microbiomes in Urban Lakes Exposed to Road Salts. Front. Microbiol. 12. doi: 10.3389/fmicb.2021.660719 Roy, S., Llewellyn, C. A., Skarstad, E., and Johnsen, G. (2011). Phytoplankton Pigments - Characterization Chemotaxonomy and Applications in Oceanography. Cambridge: Cambridge University Press.

Microgram per liter [µg/L]